Generation of a recombinant avian coronavirus infectious bronchitis virus using transient dominant selection.
Identifieur interne : 004825 ( Main/Exploration ); précédent : 004824; suivant : 004826Generation of a recombinant avian coronavirus infectious bronchitis virus using transient dominant selection.
Auteurs : Paul Britton [Royaume-Uni] ; Sharon Evans ; Brian Dove ; Marc Davies ; Rosa Casais ; Dave CavanaghSource :
- Journal of virological methods [ 0166-0934 ] ; 2005.
Descripteurs français
- KwdFr :
- ADN complémentaire (génétique), ARN viral (analyse), Animaux, Assemblage viral, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (physiologie), Infections à coronavirus (), Infections à coronavirus (anatomopathologie), Infections à coronavirus (médecine vétérinaire), Infections à coronavirus (virologie), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (physiologie), Recombinaison génétique, Techniques de culture d'organes, Virus de la bronchite infectieuse (génétique), Virus de la bronchite infectieuse (physiologie), Virus de la vaccine (génétique).
- MESH :
- analyse : ARN viral.
- anatomopathologie : Infections à coronavirus.
- génétique : ADN complémentaire, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Virus de la bronchite infectieuse, Virus de la vaccine.
- médecine vétérinaire : Infections à coronavirus.
- physiologie : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Virus de la bronchite infectieuse.
- virologie : Infections à coronavirus.
- Animaux, Assemblage viral, Glycoprotéine de spicule des coronavirus, Infections à coronavirus, Recombinaison génétique, Techniques de culture d'organes.
English descriptors
- KwdEn :
- Animals, Coronavirus Infections (pathology), Coronavirus Infections (prevention & control), Coronavirus Infections (veterinary), Coronavirus Infections (virology), DNA, Complementary (genetics), Infectious bronchitis virus (genetics), Infectious bronchitis virus (physiology), Membrane Glycoproteins (genetics), Membrane Glycoproteins (physiology), Organ Culture Techniques, RNA, Viral (analysis), Recombination, Genetic, Spike Glycoprotein, Coronavirus, Vaccinia virus (genetics), Viral Envelope Proteins (genetics), Viral Envelope Proteins (physiology), Virus Assembly.
- MESH :
- chemical , analysis : RNA, Viral.
- chemical , genetics : DNA, Complementary, Membrane Glycoproteins, Viral Envelope Proteins.
- genetics : Infectious bronchitis virus, Vaccinia virus.
- pathology : Coronavirus Infections.
- physiology : Infectious bronchitis virus, Membrane Glycoproteins, Viral Envelope Proteins.
- prevention & control : Coronavirus Infections.
- veterinary : Coronavirus Infections.
- virology : Coronavirus Infections.
- Animals, Organ Culture Techniques, Recombination, Genetic, Spike Glycoprotein, Coronavirus, Virus Assembly.
Abstract
A reverse genetics system for the avian coronavirus infectious bronchitis virus (IBV) has been described in which a full-length cDNA, corresponding to the IBV (Beaudette-CK) genome, was inserted into the vaccinia virus genome following in vitro assembly of three contiguous cDNAs [Casais, R., Thiel, V., Siddell, S.G., Cavanagh, D., Britton, P., 2001. Reverse genetics system for the avian coronavirus infectious bronchitis virus. J. Virol. 75, 12359-12369]. The method has subsequently been used to generate a recombinant IBV expressing a chimaeric S gene [Casais, R., Dove, B., Cavanagh, D., Britton, P., 2003. Recombinant avian infectious bronchitis virus expressing a heterologous spike gene demonstrates that the spike protein is a determinant of cell tropism. J. Virol. 77, 9084-9089]. Use of vaccinia virus as a vector for the full-length cDNA of the IBV genome has the advantage that modifications can be made to the IBV cDNA using homologous recombination, a method frequently used to insert and delete sequences from the vaccinia virus genome. We describe the use of homologous recombination as a method for modifying the Beaudette full-length cDNA, within the vaccinia virus genome, without the requirement for in vitro assembly of the IBV cDNA. To demonstrate the feasibility of the method we exchanged the ectodomain of the Beaudette spike gene for the corresponding region from IBV M41 and generated two recombinant infectious bronchitis viruses (rIBVs) expressing the chimaeric S protein, validating the method as an alternative way for generating rIBVs.
DOI: 10.1016/j.jviromet.2004.09.017
PubMed: 15620403
Affiliations:
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Techniques</term><term>Recombination, Genetic</term><term>Spike Glycoprotein, Coronavirus</term><term>Virus Assembly</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Assemblage viral</term><term>Glycoprotéine de spicule des coronavirus</term><term>Infections à coronavirus</term><term>Recombinaison génétique</term><term>Techniques de culture d'organes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A reverse genetics system for the avian coronavirus infectious bronchitis virus (IBV) has been described in which a full-length cDNA, corresponding to the IBV (Beaudette-CK) genome, was inserted into the vaccinia virus genome following in vitro assembly of three contiguous cDNAs [Casais, R., Thiel, V., Siddell, S.G., Cavanagh, D., Britton, P., 2001. Reverse genetics system for the avian coronavirus infectious bronchitis virus. J. Virol. 75, 12359-12369]. The method has subsequently been used to generate a recombinant IBV expressing a chimaeric S gene [Casais, R., Dove, B., Cavanagh, D., Britton, P., 2003. Recombinant avian infectious bronchitis virus expressing a heterologous spike gene demonstrates that the spike protein is a determinant of cell tropism. J. Virol. 77, 9084-9089]. Use of vaccinia virus as a vector for the full-length cDNA of the IBV genome has the advantage that modifications can be made to the IBV cDNA using homologous recombination, a method frequently used to insert and delete sequences from the vaccinia virus genome. We describe the use of homologous recombination as a method for modifying the Beaudette full-length cDNA, within the vaccinia virus genome, without the requirement for in vitro assembly of the IBV cDNA. To demonstrate the feasibility of the method we exchanged the ectodomain of the Beaudette spike gene for the corresponding region from IBV M41 and generated two recombinant infectious bronchitis viruses (rIBVs) expressing the chimaeric S protein, validating the method as an alternative way for generating rIBVs.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li></country></list><tree><noCountry><name sortKey="Casais, Rosa" sort="Casais, Rosa" uniqKey="Casais R" first="Rosa" last="Casais">Rosa Casais</name><name sortKey="Cavanagh, Dave" sort="Cavanagh, Dave" uniqKey="Cavanagh D" first="Dave" last="Cavanagh">Dave Cavanagh</name><name sortKey="Davies, Marc" sort="Davies, Marc" uniqKey="Davies M" first="Marc" last="Davies">Marc Davies</name><name sortKey="Dove, Brian" sort="Dove, Brian" uniqKey="Dove B" first="Brian" last="Dove">Brian Dove</name><name sortKey="Evans, Sharon" sort="Evans, Sharon" uniqKey="Evans S" first="Sharon" last="Evans">Sharon Evans</name></noCountry><country name="Royaume-Uni"><noRegion><name sortKey="Britton, Paul" sort="Britton, Paul" uniqKey="Britton P" first="Paul" last="Britton">Paul Britton</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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